The SEM was operated with an accelerating voltage of 15 kV and a beam current of 0.8 nA. The glass-phase content in the slag was estimated by counting the intersection points of a 200 μm mesh overlaid on back-scattered electron images. The slag samples were embedded in resin and prepared as polished thin sections that were then carbon-coated. To investigate the distribution of potentially toxic elements in the slag, the slag and lichen samples were imaged and analyzed by SEM-EDS on a JSM-6510LV (JEOL, Tokyo, Japan) and X-Max 50 (Oxford Instruments, Tokyo, Japan) detector with INCA software (Oxford Instruments, Tokyo, Japan) at Ehime University. Therefore, the present study supposes that Stereocaulon exutum has a possible practical application in biomonitoring or risk assessment of heavy metal pollution at abandoned mine sites. These chemical characteristics make this lichen a good biomarker for Cu and Zn contamination of the substrata of the lichen. The Cu and Zn concentrations in the thalli are positively correlated with the concentrations in the corresponding substrata and a positive intercept in the regression curve obtained using a linear function. The others are distributed on the surface of the hyphae as formless particles and show lateral distribution inside the cortex of the thallus. Some of these dissolved elements are distributed in the cells of the hyphae. The dissolved elements are absorbed into the lichen thallus. The heavy metals and As in the slag are dissolved from their original phases during the weathering process by lichen substances (organic acids) and hypha penetration, in addition to non-biological weathering. One-time license fee for play on account’s designated primary PS4™ system and other PS4™ systems when signed in with that account.The lichen, Stereocaulon exutum Nylander, occurring in a contaminated abandoned mine site was investigated to clarify (1) the behavior of heavy metals and As during the slag weathering processes mediated by the lichen and (2) the distribution of these elements in the lichen thallus on slag. Software subject to license (us./softwarelicense). Enhanced game play on PS4 Pro console Optional In-game purchases Online play required Online features require an account and are subject to terms of service and applicable privacy policy (/terms-of-service & /privacy-policy). Online features require an account and are subject to our terms of service (/terms-of-service), our privacy policy (/privacy-policy), and the game publisher’s privacy policy. Rest assured that there is no way to buy in-game items that give vehicles gameplay advantages. PLAY TOGETHER Meet new players in the game or bring your friends for way more fun! MAY THE BEST GLADIATOR WIN HMM is free-to-play and not pay-to-win. COMPETITION If you feel competitive, you can participate in Community Tournaments, or even the Metal League, Heavy Metal Machines' Official Tournament, that features cash-prizes to fuel the game's Esports scene. NEWCOMERS ARE WELCOME If you are new to HMM, no worries! Training modes are available to sharpen your skills. BECOME A METAL GLADIATOR You can play HMM casually, or if you want to spice things up, join the Ranked Mode to be godlike, or create your Team and bring it to the Colosseum, an in-game Tournament, to battle for prizes and prestige. A ROSTER OF UNIQUE VEHICLES Ranging from a car crushing Monster Truck and a heavy armored Tank to a blade thrower Motorcycle and a magnetic Tow Truck, each of the many vehicles has a different set of weapons and gameplay styles that bring a wide range of strategic possibilities to each match. A WHOLE NEW EXPERIENCE HMM's combination of unique vehicles, fast-paced combat, and post-apocalyptic sports arenas will take you to a whole new gameplay experience. PlayStation® Plus subscription is NOT necessary to play HMM. Heavy Metal Machines is a one of a kind Free-to-Play Multiplayer Vehicular Combat game where players engage in 4v4 intense battles, controlling lethal vehicles in post-apocalyptic arenas. Although this game is playable on PS5, some features available on PS4 may be absent. To play this game on PS5, your system may need to be updated to the latest system software.
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